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KMID : 0603820070130040325
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Journal of Experimental & Biomedical Science
2007 Volume.13 No. 4 p.325 ~ p.332
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Mycobacterium tuberculosis Derived Epitope Peptide Specific CD8+T Cell Responses in Tuberculous Pleurisy
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Cho Jang-Eun
Kim Young-Sam
Park Moo-Suk
Lee Kyung-Wha
Cho Sang-Nae
Cho Sung-Ae
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Abstract
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Cell-mediated immune response (CMI) is a major immune protective mechanism against tuberculosis (TB) infection. Among several components involved in CMI, recent studies suggest that CD8+ T cells are important in controlling TB infection. In our previous report, we defined four Mycobacterium tuberculosis (MTB) derived epiotpe peptides specific for HLA-A*0201-restricted CD8+ T cells. These four peptides are PstAl_{75-83}, ThyA_{30-38}, RpoB_{127-135} and 85B_{15-23}. In this study, these epitope peptides specific CD8+ T cell responses in tuberculous pleurisy were investigated using ex vivo IFN-gamma elispot assay and intracellular IFN-gamma staining method. As a result, we observed these epitope peptide specific CD8+ T cell responses are induced in all three patients with tuberculous pleurisy suggesting that CD8+ T cells are involved in protective immune mechanism against MTB infection in tuberculous pleurisy. However, the CMI to mitogens and MTB antigens from pleural fluids of patients with tuberculous pleurisy does not seem to correlate with that from peripheral blood, although the sample size is too small to make any conclusion. In sum, the MHC I restricted CD8+ T cell responses seem to be induced efficiently in the pleural fluids, at the site of TB infection, in which the CMI is actively induced. In addition, these experiments suggest that MHC I restricted CD8+ T cell mediated immune responses are also involved in protective mechanism against MTB infection in extra-pulmonary TB.
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KEYWORD
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CD8+ T cells, Tuberculosis (TB), Mycobacterium tuberculosis (MTB), Tuberculous pleurisy, Pleural fluid mononuclear cells (PFMC), Cell mediated immune response (CMI)
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